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Definitions are the foundation for any scientific work, but with a significant increase in publication numbers, gathering definitions relevant to any keyword has become challenging. We therefore introduce SciDef, an LLM-based pipeline for automated definition extraction. We test SciDef on DefExtra & DefSim, novel datasets of human-extracted definitions and definition-pairs' similarity, respectively. Evaluating 16 language models across prompting strategies, we demonstrate that multi-step and DSPy-optimized prompting improve extraction performance. To evaluate extraction, we test various metrics and show that an NLI-based method yields the most reliable results. We show that LLMs are largely able to extract definitions from scientific literature (86.4% of definitions from our test-set); yet future work should focus not just on finding definitions, but on identifying relevant ones, as models tend to over-generate them. Code & datasets are available at this https URL.